Multiphoton Microscopy Standard Wavelengths
Conoptics manufactures a range of laser accessories designed specifically for Multiphoton Microscopy (MPM), including laser modulation systems, driver electronics, beam deflectors, noise reduction tools, and optical isolators. As a leader in the field, Conoptics has developed specialized solutions that optimize the performance of MPM systems, ensuring precision and reliability for advanced imaging applications.
Multiphoton microscopy (MPM) is a powerful research technique that combines advanced laser scanning microscopy with long-wavelength multiphoton fluorescence excitation. This approach enables MPM to capture high-resolution, three-dimensional images of specimens tagged with highly specific fluorophores.
Conoptics’ Model 350-80LA with the BK (resonance-dampened) option, part of the KD*P Series Electro-Optic modulator, is an ideal solution for multiphoton microscopy (MPM) systems. Paired with the Model 302RM amplifier, it offers precise control over laser intensity and high-speed shuttering. Additionally, this setup manages beam attenuation and fly-back blanking with minimal dispersion, providing full modulation across the laser’s bandwidth. The system operates without spatial dispersion and achieves rise/fall times of 1 microsecond, making it highly effective for MPM applications.
Model 350-80LA Electro-Optic Modulator
| Aperture | 3.5mm |
| Dimensions | 50mm Diameter x 137mm |
| Transmission | >90% |
| Standard Wavelength Ranges | 700-to-1100nm |
| Piezo Resonances | Minimal with clamped version (BK option) |
| Volts ½ wave at 830nm | 600 Volts |
| Contrast Ratio | 500:1 |
| Driver Compatibility | Full modulation with M302 Power Amplifier up to 1064nm |
Model 302RM Driver
| Cabinet | Driver and power supply in singe cabinet |
| Test Feature | Built-in test feature allows testing for max transmission of Pockel-Cell without adjusting bias voltage |
| Input Impedance | Choice of amplifier input impedance by rear panel switch (50ohm/1k ohm) |
| DC Bias | Improved DC biasing of Pockel-Cell provides greater linearity at higher bias voltages |
| Voltage Range | +/- 450VDC controlled by ten-turn front panel pot. Digital meter monitors differential bias applied to E.O. Modulator |
| Linearity | 10 bits referenced to full scale (.1%) |
| Bandwidth | DC to >200Khz with 90pf load and 3M (RG-62) cables |
| Max. Output Drive Level | 750VP-P into 90pf load |
| Amplifier Input Signal | 2VP-P max into 50/1k ohms delivers 750VP-P out |
| Input Power (AC) | 60W typical. Input power is both load (modulator) and frequency dependent. |
| Dimensions | 19″ Rack Mountable, 5.25″ H(133mm) (3U) x 14″D (356mm) |
| Cooling | Forced air |
| Operating Environment | Designed for laboratory use (indoor only) Temperature range +5deg C to +50 deg C ambient Humidity 20%-80% RH up to 32deg C Altitude <3000M |
| Weight | 20lbs (9.07kg) |
Output Response
The image below provides the detected response at 514nm, 3ms pulse width, 250Hz rate
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